ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from Hypericum perforatum.</p><p><b>METHOD</b>Compouds were isolated by chromatographic techniques. Their structures were identified by spectral methods. The inhibitory activity of recombinant human PTP1B was evaluated.</p><p><b>RESULT</b>Nine compounds were elucidated as D-Mannitol (1), 1,2-benzenedicarboxylic acid bis(1-methylpropyl) ester (2), (7E, 6R,9S)-9-hydroxy-4,7-megastigmadien-3-one (3), (6S,9R)-roseoside (4) , 2,6-dimethoxy-4-hydroquinone-l-O-beta-D-glucopyranoside (5), 2,6-di-methoxy-4-hydroxybenzyl alcohol 1 -O-beta-D-glucopyranoside (6), syringate 4-O-beta-glucopyranoside (7), hypericin (8), skyrin-6-0-beta-D-glucopyranoside (9) , (R)-2,3-dihydroxypropyl 3,4-dihydroxy-benzoate (10). At a concentration of 2 micromol x L(-1), compound 8 inhibited recombinant human PTP1B with inhibitory rate of 96.4% and IC50 of 2.5 micromol x L(-1).</p><p><b>CONCLUSION</b>compound 10 was new, compounds 2-4, 7 were obtained from Hypericum for the first time and compounds 5-6 was isolated from this plant for the first time. Compound 8 showed remarkable PTP1B inhibitory activity.</p>
Subject(s)
Anthraquinones , Chemistry , Drugs, Chinese Herbal , Chemistry , Glucosides , Chemistry , Hypericum , Chemistry , Molecular Structure , Norisoprenoids , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of aerial parts of Ammopiptanthus mongolicus.</p><p><b>METHOD</b>Isolation and purification were carried out on silica gel, Sephadex LH-20 and HPLC column chromatography. The structures of the compounds were identified by physico-chemical properties and spectral analysis.</p><p><b>RESULT</b>Nine compounds were isolated and identified as (+)-maackiain (1), brevifolin (2), 7-hydroxy-4'-methoxy isoflavanone (3), daidzein 4',7-diglucoside (4), genistein 4', 7-di-O-beta-D-glucoside (5), isolupalbigenin (6), ononin (7), beta-sitosterol (8), beta-daucosterol (9).</p><p><b>CONCLUSION</b>Compounds 2, 4 - 6 were obtained from the genus Ammopiptanthus for the first time.</p>
Subject(s)
Chromatography, Agarose , Methods , Chromatography, High Pressure Liquid , Methods , Fabaceae , Chemistry , Glucosides , Chemistry , Isoflavones , Chemistry , Plant Extracts , Chemistry , Plant Leaves , Chemistry , Pterocarpans , Chemistry , Silica Gel , Sitosterols , Chemistry , Taxoids , ChemistryABSTRACT
The chemical constituents of Parthenocissus quinque were investigated. The chemical constituents were isolated by column chromatography on silical gel and sephadex LH-20. Their structures were elucidated on the basis of spectral analysis and of comparison of physical constant. Nine compounds were isolated from this plant and the structures of them were identified as 3,4,5-trihydroxy- benzoic acid (1), piceatannol (2), resveratrol (3), resveratrol trans-dehydrodimer (4), cyphoste mmin B (5), pallidol (6), cyphostemmin A (7), quercetin-3-O-alpha-L-rhamnoside (8), myricetin-3-O-alpha-L-rhamnoside (9), respectively. Compounds 1, 4-9 were isolated from this plant for the first time.